PDK1 antibody orb345401 from Biorbyt

Product Specs
ItemPDK1 antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb345401
Quantity100 µg
ApplicationsELISA, IHC, WB
Conjugate/TagUnconjugated
PurityThis affinity purified antibody is directed against human PDK-1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with PDK-1 protein from mouse, rat and dog based on 92% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown
TargetPDK1
IsotypeIgG
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human PDK-1 protein
NCBI Full Gene Name3-phosphoinositide dependent protein kinase 1, pyruvate dehydrogenase kinase 1
ClonalityPolyclonal
HostRabbit
Concentration1.3 mg/mL
ReactivityHuman
FormatLiquid (sterile filtered)
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use
NCBI Gene AliasesPDK1, PDPK2, PDPK2P, PRO0461

Specifications/Features

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kDa in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown.

Application Dilutions: ELISA: 1:100,000, IHC: User Optimized, WB: 1:500 - 1:2,000

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